Research Technician

South San Francisco, CA 94080

Posted: 09/25/2018 Employment Type: Contract Industry: Research - Discovery Job Number: 4862

Our client is a leading biotechnology company that discovers, develops, manufactures and commercializes medicines to treat patients with serious or life-threatening medical conditions. They are among the world' s leading biotech companies, with multiple products on the market and a promising development pipeline.

 

Duties:
  • Molecular cloning of expression or genome editing vectors
  • Contruction of vectors using restriction cloning, PCR cloning, and/or gene synthesis technology.
  • Plasmid DNA prep and sequence analysis.
  • Genomic DNA and mRNA preparation 
  • From cultured mammalian cells.
  • PCR or qRT-PCR
  • Analysis of genomic DNA or mRNA. Primer design and optimization.Cell culture
  • Passaging and counting of cultured mammalian cells. Lipid based transfection and/or electroporation of cells.
  • Single cell cloning.

 

Skills:

  • Excellent skills in communication, organization, flexibility and an eagerness to learn.
  • Must work well with others and be a team player.
  • Experience with molecular cloning, nucleic acid extraction, purification, and analysis (qualitative and quantitave assessment of DNA and RNA), and mammalian cell culture (sterile technique, cell counting, and passaging).
  • Direct experience with RNA interference, CRISPR, genome editing, and/or expression vector design and cloning is a plus.

 

Education:

  • Bachelors or Masters degree in a biology-related field (Biotechnology, Cell Biology, Molecular Biology, or Biochemistry preferred) with 2+ years of laboratory research experience or PhD in biomedical field with 0-5 years experience
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